Czech Republic v Portugal
Rosicky, 31, has been struggling to be fit in time after suffering an inflamed Achilles tendon in the first-half of the 2-1 group stage win over Greece which saw him miss the final Group A game, the 1-0 win over co-hosts Poland.
However, whilst he was able to train on Wednesday for the first time in over a week after undergoing two days of treatment in Prague, Smicer said his teammate from the flair-filled Euro 2004 side was not fit enough to start.
"It is certain that Tomas will not play from the start," he told Czech public TV channel CT.
However, Arsenal star Rosicky, who has been plagued by injuries throughout his career, one of which forced him to miss Euro 2008, will be on the substitutes' bench and could get some playing time, according to Smicer.
The quarterfinal will be the third meeting between the two sides in five Euros, both winning one apiece, though, the Czechs won the most important one the 1996 quarterfinal 1-0 on their way to losing to Germany in the final.